The Law Of The Gods - 1731 Words

Antigone Essay The big question many face is whether the law of man is more important than the law of the gods. In the play Antigone Creon has a hard time giving up the law of man to submit to the law of the gods, and in contrast Antigone undertakes the problem of deciphering between what she believes the gods want, and what truly is there will. Despite the noble intentions of Creon and Antigone they fall prey to hubris and irrationality resulting in their disregard for the gods, which ultimately leads to their fall from grace. Creon strongly believes that preserving the state of Thebes, and protecting the people he governs is above all else. Creon’s belief is that to assert his new dominance over the people of Thebes he has to overrule the law of the gods which emphasizes the idea that his law is strong and cannot be broken. He demonstrates this when he refuses to allow anyone to bury the body of Polyneices, who committed crimes against his own city. Creon shows nobility with his love for his people, and also his respect in man-made law, but his nobility is overshadowed because he is contradicting the laws of the gods. As the novel comes to a close he is severely punished for his disregard of the gods will and is sentenced to knowing his wife and son took their own lives because of him. Creon realizes that the only way to reconcile with the gods is to keep living and bask in his own regret for what he brought upon himself and his family. The act of refusing to commitShow MoreRelatedThe Law And The J udgement Of God1930 Words   |  8 PagesWhat is the law? Does it provide the necessary soteriological provision for those that uphold it over the privileges found in faith? The Apostle Paul, who at one time was a staunch supporter of this view of the law before his conversion now seeks to show the truth of what the Mosaic Law has done and what its functions are in the age of the Messiah. Paul’s argument of justification by faith is stacked against the legalistic points of the traditions of the Mosaic Law and what it is that providesRead MoreObey the Laws of God, Not the Laws of Man2572 Words   |  11 PagesObey the Laws of God, Not the Laws of Man On Monday, April 11th 2011, France became the first country to put into force a law that bans Muslim women wearing the Burqa in any public setting. Not only did this ban create a global debate, a domino effect on other countries to put forth the same law, but also creating tension among French Muslims and their citizens (Ulusoy). The Quran asks believing women to cover although Muslim women interpret covering their bodies in different ways. The Burqa coversRead MoreLaws s Of The Good God1857 Words   |  8 PagesChallenge Accepted: Laws’s refutation of the â€Å"Good God† The English philosopher Stephen Law’s â€Å"The evil-god challenge† is a retort against the arguments raised in favour of the theistic belief that the world was created and is governed by an all knowing, supremely benevolent being. In order to refute the existence of the notion of a â€Å"good-god† as constructed in the arguments of theists, law has constructed his own hypothetical entity the â€Å"evil-god†. Law’s challenge stems from the logical and evidentiaryRead MoreThe Evil God Challenge : Stephen Law1561 Words   |  7 PagesEvil God Challenge Introduction Stephen Law conducted a thought experiment with a purpose of establishing the existence of an evil God, whereby he challenged those who believed in the presence of a kind and good God, doing nothing evil, and argued that the existent God is wicked indeed. The hypothesis developed into the challenge based on the argument that, if an omnibenevolent God is said to exist, yet there is so much evil in the world, then there is as well a possibility that an evil God existsRead MoreThe Theory Of God s Eternal Law1382 Words   |  6 Pagesstructure of reality by God, who is a subsistent being. Since God is a subsistent being, all creatures, including humans, are â€Å"placed by him in existence† and they are therefore naturally oriented towards him. Because of this, â€Å"natural law is therefore defined as a participation in the eternal law,† and it is mediated by human reason and human inclinations—which participate in the divine intelligence. These inclinations enab le people to participate in God’s eternal law because their nature hasRead MoreGod s A Perfect Law Of Liberty1337 Words   |  6 PagesFurther evidence can be found throughout the Bible to support that the â€Å"perfect† is referring to the New Testament. In James 1:25, God’s word is referred to as the â€Å"perfect law of liberty†. The New Testament contains â€Å"all things that pertain to life and godliness† (2 Peter 1:3), which shows that it is perfect and complete. God’s word is capable of making man â€Å"complete† or â€Å"perfect† (2 Timothy 3:16, 17). Another context that is very similar to 1 Corinthians 13:8-13 is Ephesians 4:7-16, which helpsRead MoreStephen Law : Evil God Challenge1897 Words   |  8 PagesStephen Law: Evil-God-Challenge Introduction English philosopher Stephen Law is attached to Heythrop College of University of London as a senior lecturer. As an acknowledged atheist Stephen Law in his argument Evil-God Challenge challenged the theists. He said that evidence shows existence of Evil negates the idea of a Good-God. He pre-empts the arguments of theodices by this cryptic phrase â€Å"Evil-God Challenge†. By it the theists are asked if there is anyone who thinks that there is an Evil God. ThenRead MoreTheories Of God s Moral Law1818 Words   |  8 Pagestheories of God’s moral law, antinomianism believes there is â€Å"no binding moral laws, that everything is relative† (Geisler, 2010, p. 22). Although this ethical theory has produced historical rebellions that were required at that point in time, such beliefs are, â€Å"indeed quite literally an-archy, an unfettered and unconditional rejection of all ruling principles in the name of freedom† eventually also known as chaos (Smith, 2000, p. 130). Without any type of moral law, there would be no order leadingRead More Great Expectations: Gods Law Vs. Human Law Essay1164 Words   |  5 Pages Great Expectations: Gods Law vs. Human Law In his book Great Expectations, the problematic nature of moral judgement and justice that stems from a conflict between Gods law and human law is one of several topical themes that Charles Dickens addresses. This paradox regularly surfaces in his treatment of plot and setting, and is more subtlety illustrated in his use of character. To facilitate the readers awareness of such a conflict, the narrator often uses language that has Christian connotationsRead MoreRelationship Between Obedience And The Law And Gods Blessing931 Words   |  4 Pages Introduction Throughout our lives obedience to Gods will in all areas of our life can be a struggle. The people of ancient Israel stood as testament to this. However Gods promise to the Israelites is seen in this passage promising his blessings if they stood true to his law. This essay will explore the relationship between obedience to the law and Gods blessing using Jacqueline Grey’s Them, Us Me principle of looking at the bible. Them The book of Deuteronomy was written while Israel was in

Air France Financial Analysis - 1289 Words

Introduction to Finance Air France - KLM Financial Analysis. Before reading I chose Air France as a company to analyze it financially-speaking. But, as my major is in economy in ENPC, I will also include strategic information in this report. I based the financial accounting on the data provided by Yahoo! Finance, which are the three statements, back to March 31, 2007 for the oldest report base. I also reviewed some of other companies in the same business line, especially for main indicators (EBIT, ROC, ROA, P/E...). It will be mentioned when done so. 1. Introduction Air France-KLM is one of the major airline companies, and results of the merge of Air France and KLM in 2005. As for any†¦show more content†¦We can expect better | | | | |afterwards. | |3.Fin. Structure |Debt Ratio |0.85 |Pretty good, valuable assets | | |Debt-to-Equity |171.1% |Seems bad, but normal for business line, | | |Total Liabilities/Total Assets |77.6% |(high needs). | |4. Asset Mgmt |Inventory Turnover |34.2% |Good. Shows previous good management. | | |Total Assets Turnover |0.9 | | |5. Various |EBITDA Margin |6.17% |Acceptable. | | |Return On Assets |-0.29% |Compared to 2007 years, low, but in this | | |Return OnShow MoreRelatedAirFrance-KLM Report Essay example1345 Words   |  6 Pagesz CASE STUDY: Air France – KLM: Changing the Rules of the Game [Type text] Introduction Air France-KLM Case (Som 2009) provides the background for airlines industry and factors impacting companies’ positions, details about the history of air-carrier alliances and their challenges. The main focus of the Case is on two companies: Air France and KLM and their decision to merge despite predictions of failure. The period covered by the case ends in 2006. As most aviation companies worldwideRead MoreAir France Case998 Words   |  4 PagesAir France Case Brief Executive Summary Rob Griffin, the senior vice president and U.S. director of search for Media contacts, a media consulting firm, is faced with the task of optimizing search engine marketing (SEM) for Air France as the company seeks to compete in the hyper-competitive U.S. market. Even though Griffin is satisfied with the performance of his company, he wants to make the team to remain the leading position and provide the results that Air France wanted. At the time of theRead MoreThe Organizational Change And Development Of A Travel And Tourism Organization933 Words   |  4 PagesManagement for Travel and Tourism Name: BALKISU TEJAN, ID: 37007 ANALYSIS OF THE ORGANISATIONAL CHANGE AND DEVELOPMENT OF A TRAVEL AND TOURISM ORGANIZATION: CASE STUDY OF BRITISH AIRWAYS It is essential that an organization undergoes growth and development, Organizational change and innovation. 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The internal analysis 5 1.1 Historical overview 5 1.2 Mission, vision, goals and strategy 5 1.3 Structure 6 1.3.1 Corporate Governance and management control systems 6 1.3.2 Leadership 6 1.3.3 Culture 7 1.3.4 Structural configuration 7 2. The external analysis 8 2.1 Current situation 8 2.1.1 SWOT analysis 8 2.1.2 BCG matrix 9 2.2 In relation to the competitionRead MoreAirbus Marketing Plan1019 Words   |  5 PagesEuropean airspace company 1 Marketing Plan - Agenda 1. Strategic analysis 2. Achievable Marketing Objectives for 2010 3. Yearly Action Plan Marketing Budget 4. Control Procedures Criteria of Success 2 Marketing Plan - Agenda 1. Strategic analysis 2. Achievable Marketing Objectives for 2010 3. Yearly Action Plan Marketing Budget 4. Control Procedures Criteria of Success 2 Strategic analysis 1.1 Business Trend Aviation is an important element in today’s world andRead MoreQuantitative And Quantitative Risk Analysis788 Words   |  4 PagesQuantitative Risk Analysis - Ebony The quantitative risk analysis is a method for further evaluating the effect of proposed risks identified by project stakeholders and teams on the ability to achieve project objectives (Heldman, 2013, p. 274). Project teams use methods including sensitivity analyses or predictive modeling and simulation techniques to determine how the risk will affect project progress (Heldman, 2013, pp. 276,278). During the quantitative analysis processes, each risk is rated basedRead MoreEssay on Harvard Case Study - Sealed Air Corporation1675 Words   |  7 Pages1.0 INTRODUCTION The Sealed Air corporation is committed to market leadership through technological innovation. Ten years ago, the company was first to market with a highly successful coated air-bubble packaging protection product, AirCap. However, market trends indicate a rapid displacement of coated bubble by a technologically inferior yet inexpensive uncoated product. Burgeoning demand for uncoated bubble poses a direct threat to the long-term viability of the technologically superior, premiumRead MoreSealed Air Coporation Case Study Essay1735 Words   |  7 Pages1.0 INTRODUCTION The Sealed Air corporation is committed to market leadership through technological innovation. Ten years ago, the company was first to market with a highly successful coated air-bubble packaging protection product, AirCap. However, market trends indicate a rapid displacement of coated bubble by a technologically inferior yet inexpensive uncoated product. Burgeoning demand for uncoated bubble poses a direct threat to the long-term viability of the technologically superior, premiumRead MoreThe Influence of the Plague on the Church671 Words   |  3 PagesIn the wake of the plague, nobleman and religious authority figures struggled to maintain their high place in society as government leaders were unable to find an effective response to the disease, and limiting the allotment of the financial growth of the lower class, preventing them from gaining societal power, as well as increased suspicion concerning the power of the Church. Confusion and suspicion arose among followers of the church when the Church was powerless to stop this devastating diseases

Being Wrong Chapter 5 Free Essays

Samantha Merlin October 14, 2012 IGE 120 MWF 11:45-12:50 Reading Response: Being Wrong: Chapter 5 by Kathryn Schultz At the beginning of chapter 5 â€Å"Our Minds, Part Two: Belief† Schultz informs us that Alan Greenspan testifies before congress on October 23, 2008 because of the financial crisis. The chapter then leads on to expand on theâ€Å"Greenspan moments† which is basically when beliefs fail us. Belief in casual conversation is a conscious belief, such as morality, politics, ourselves or others. We will write a custom essay sample on Being Wrong Chapter 5 or any similar topic only for you Order Now Philosophers include all unconscious beliefs too, like believing that the sky is dark outside if you’re in your bedroom at night with the blinds closed and that the sun won’t rise for many more hours and when it does it will do so in the east. Both explicit beliefs like â€Å"everyone hates me† and implicit ones â€Å"the sky is blue† serve as a function of helping me figure out where to sit when I enter a room. Once an implicit assumption is violated, it becomes explicit. If I suddenly fall through the floor, my implicit assumptions about the solidity of the floor suddenly appear in my conscious. The beliefs at the acute ends of the implicit and explicit range breaks down most strikingly when they are revealed incorrectly. However, holding a belief can have many outcomes. Belief in overall perspective led to spending $300 million and $30 million per year on LIGO. We have distal beliefs because we need to be able to theorize about some things, but end up theorizing about everything. The theorizing process is quick and automated and doesn’t require us to intentionally activate it, so we cant stop theorizing. We tend to mainly notice our theories when they’re wrong. Babies as young as seven months are already theorizing about gravity. Alison Gopnik assumed that the theory drive exists particularly esfor early childhood, but functions throughout lives, just like sex drive exists precisely for fertile years, but works before and after. Although we are good at making theories, we are not good at realizing we made them. We have a tendency toward â€Å"Naive realism† which means that our perception matches reality. Anyhow, this can not be true because there are things that we can not perceive like infrared and molecules. All children under the age of four are Naive realists because they believe that we can not believe things that are wrong. The chapters main idea was that if you believe that your beliefs are true, you will assume that those that disagree with you are ignorant of facts, are idiots or evil. Just from reading this chapter I have come to the conclusion that Schulz takes on a heavy topic that most of us don’t understand. The broad majority of people either feel they have to be right at all costs, or that being wrong is a personal failure. In reality, being wrong is what helps us grow and understand our world better. It was impossible not to think about politics while reading this, either. Each political party has a hard and fast set of beliefs that define them, and anyone not advocating to those beliefs is wrong and needs to be corrected. Unluckily, even when presented with evidence to the contrary relating to one of their closely-held views, it is nearly impossible for the person to adjust their thinking and admit they were wrong. There’s no discussion and consideration of views to come up with a compromise or to learn from others. It’s often a duel to the death to be right while proving the other person wrong. In the end, nobody gains from that. In class i’d like to discuss whether people thought that Being Wrong had a great message and can make a difference in someone’s life or thought it can’t cause any type of dramatic change of perspective. I feel that it can make a person’s life more productive and enjoyable. Unfortunately, I have little hope that the average man on the street who is affected by the need to always be right will ever take the time to read and understand the message of this chapter or the whole book in general. How to cite Being Wrong Chapter 5, Papers

The Poets Audience free essay sample

An analysis of poetry and its intended audience. This paper discusses three poems composed by three different poets, Wordsworth, Springsteen and Hughes. Through these works, the writer examines who the poet addresses. This paper illustrates how every poem has a dual audience for the thoughts expressed, with a primary entity being addressed in the poem itself, and with the reader a secondary entity addressed by the poem as a whole. Poetry is a personal expression of thought and emotion, and the issue is raised concerning to whom the poet addresses the poem. Every poem has a dual audience for the thoughts expressed, with a primary entity being addressed in the poem itself, and with the reader a secondary entity addressed by the poem as a whole. This may be more obvious in a dramatic poem like those written by Robert Browning, where the person addressed is clearly identified, but the poet always addresses someone even if this is simply implied. We will write a custom essay sample on The Poets Audience or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page

Can a good dissertation increase your employability?

For most students the prospect of undertaking a dissertation is a daunting one. University guidelines state that the body of a dissertation can range from ten to twenty-five thousand words, depending on the qualification. This is a substantial undertaking for anyone. Let's imagine that you're a dedicated student. You're studious, achieving above average marks, but not likely to be invited to University Challenge any time soon. You've worked your way through the first two years and now, in the final year of your degree, the prospect of completing a body of work that counts towards a substantial portion of your final grade is fast looming on the horizon. To compound that unnerving feeling in the pit of your stomach, you've also come to the stark realisation that you're CV is hardly bursting at the seams with relevant work experience, transferrable skills that prove invaluable in employment, let alone an army of contacts to call on when you finally graduate and hit the job market. How are you to prove to employers that you have that edge over any other prospective candidates? Do you need to make a positive pre-interview impression to get your foot in the door? Is the accumulated debt over the course of your education weighing heavy on your mind, and you need to secure employment quickly? An eloquent dissertation can dazzle employers, and secure you that much needed position. The ability to write clear and accurate text is the most useful skill that you will learn at university. Whatever subject you specialise in, and whatever career you choose after you graduate, a command of language is a valuable asset. When employers offer a job to an MA graduate they are sometimes interested in how much he or she knows about Charles Dickens or the Napoleonic wars, but they are always looking for someone with good communication skills and an eye for detail. In almost any job, you will spend time working with a range of texts. You may produce written reports, letters or marketing copy. You may also give lectures or presentations. If you are aiming for a career in which you can use language stylishly, such as journalism or creative writing, it is equally important that you know the rules of good plain English. It's true that summer internships, placements and graduate schemes are still effective ways of procuring employment, but in today's competitive job market it's highly advisable to have as many strings to your bow as possible. It's also true that not all employers will base their selection of candidates on the strength of their dissertation. However, those candidates who have demonstrated their ability to complete such a demanding project to the very highest of standards will surely mark themselves out as the

Free Essays on For Whom the Bell Tolls

For Whom the Bell Tolls begins and ends in a pine-scented forest, somewhere in Spain. The year is 1937 and the Spanish Civil War is in full swing. Robert Jordan, a demolitions expert attached to the International Brigades, lies "flat on the brown, pine-needled floor of the forest, his chin on his folded arms, and high overhead the wind blew in the tops of the pine trees." The sylvan setting, however, is at sharp odds with the reason Jordan is there: he has come to blow up a bridge on behalf of the antifascist guerrilla forces. He hopes he'll be able to rely on their local leader, Pablo, to help carry out the mission, but upon meeting him, Jordan has his doubts: "I don't like that sadness, he thought. That sadness is bad. That's the sadness they get before they quit or before they betray. That is the sadness that comes before the sell-out." For Pablo, it seems, has had enough of the war. He has amassed for himself a small herd of horses and wants only to stay quietly in the hil! ls and attract as little attention as possible. Jordan's arrivaland his missionhave seriously alarmed him. "I am tired of being hunted. Here we are all right. Now if you blow a bridge here, we will be hunted. If they know we are here and hunt for us with planes, they will find us. If they send Moors to hunt us out, they will find us and we must go. I am tired of all this. You hear?" He turned to Robert Jordan. "What right have you, a foreigner, to come to me and tell me what I must do?" In one short chapter Hemingway lays out the blueprint for what is to come: Jordan's sense of duty versus Pablo's dangerous self-interest and weariness with the war. Complicating matters even more are two members of the guerrilla leader's small band: his "woman" Pilar, and Maria, a young woman whom Pablo rescued from a Republican prison train. Unlike her man, Pilar is still fiercely devoted to the cause and as Pablo's loyalty wanes, she becomes the moral center of the grou... Free Essays on For Whom the Bell Tolls Free Essays on For Whom the Bell Tolls For Whom the Bell Tolls begins and ends in a pine-scented forest, somewhere in Spain. The year is 1937 and the Spanish Civil War is in full swing. Robert Jordan, a demolitions expert attached to the International Brigades, lies "flat on the brown, pine-needled floor of the forest, his chin on his folded arms, and high overhead the wind blew in the tops of the pine trees." The sylvan setting, however, is at sharp odds with the reason Jordan is there: he has come to blow up a bridge on behalf of the antifascist guerrilla forces. He hopes he'll be able to rely on their local leader, Pablo, to help carry out the mission, but upon meeting him, Jordan has his doubts: "I don't like that sadness, he thought. That sadness is bad. That's the sadness they get before they quit or before they betray. That is the sadness that comes before the sell-out." For Pablo, it seems, has had enough of the war. He has amassed for himself a small herd of horses and wants only to stay quietly in the hil! ls and attract as little attention as possible. Jordan's arrivaland his missionhave seriously alarmed him. "I am tired of being hunted. Here we are all right. Now if you blow a bridge here, we will be hunted. If they know we are here and hunt for us with planes, they will find us. If they send Moors to hunt us out, they will find us and we must go. I am tired of all this. You hear?" He turned to Robert Jordan. "What right have you, a foreigner, to come to me and tell me what I must do?" In one short chapter Hemingway lays out the blueprint for what is to come: Jordan's sense of duty versus Pablo's dangerous self-interest and weariness with the war. Complicating matters even more are two members of the guerrilla leader's small band: his "woman" Pilar, and Maria, a young woman whom Pablo rescued from a Republican prison train. Unlike her man, Pilar is still fiercely devoted to the cause and as Pablo's loyalty wanes, she becomes the moral center of the grou...

How do humans interfere with biogeochemical cycles Essay

How do humans interfere with biogeochemical cycles - Essay Example This nitrogen then travels to animals and humans when the plants are eaten, and is excreted as urine, which contains ammonia. Ammonia is poisonous, but autotrophic or nitrifying bacteria have the ability to oxidize it into nitrites and nitrates, which is the form of nitrogen that is most usable for plant metabolism.(CSuros, 1999:61). Similarly denitrifying bacteria have the ability to reduce nitrates to nitrous oxide and nitrogen, which can then be released back into the atmosphere. All of the above activities disturb the natural balance of nitrogen in the atmosphere and present within the soil. Human beings have interfered with the natural progress of the nitrogen cycle by dumping large amounts of untreated sewage into water bodies, which when coupled with the urban runoff has produced an overload of nitrogen in aquatic systems. The net result of this excessive nitrogen in water is eutrophication, or the excessive growth of algae that in turn draw out all the oxygen in the water, so that other forms of marine life, such as fish, are unable to survive. (www.library.thinkquest.org). This also upsets the delicate, natural ecological balance of nitrogen that is maintained through the nitrogen cycle, which generally ensures the amount of nitrogen in the atmosphere at about 79%. When eutrophication persists, then it produces a phenomenon referred to as the red tide, wherein the lack of oxygen in the water ca stifle and kill off any forms of life in the water. Atmospheric nitrogen is used by plants and the nitrogen excreted through the urine of plants and animals is fixed back into nitrates and nitrites using the nitrifying bacteria. But when excessive nitrogen is released through the dumping of too much sewage, it complicates issues by reducing the oxygen levels and thereby disturbing the balance of atmospheric gases as well. Since oxygen is necessary for living beings to survive and the percentage of atmospheric oxygen needs to be maintained in order to

Answer 9 questions Essay Example | Topics and Well Written Essays - 1500 words

Answer 9 questions - Essay Example different language because in most cases, word pronunciation makes a great deal in bringing our messages across no matter how wide one’s vocabulary is in a certain language. Sound production is a widely recognized problem and considered to be the most challenging part in learning a second language. Intonation and accentuation are equally challenging but do not give much problems in contrast to sound production because it is the pronunciation of the word correctly that makes one coherent. Older learners have difficulty in achieving a native-like pronunciation because they have already been used to the accent and intonation of their own tongue. This may be compared to training a plant to follow a bend that the florist desires for it to have a unique design wherein, if it is still young, the plant is easily bent while when it is already of age with its trunk hardened, there would be difficulty in bending it. Thus, if one wants to learn another language, it is best to start while he is still young and is able to develop the communication skills more effectively. Pronunciation should be taught not only in a certain period of time where the lesson is concentrated in sound formation but should be taught in all levels of the teaching process. Correction is more effective when the mistake is brought to light right then and there because the student is most aware of the mistake when he just made it. Whereas, when you let a mistake pass and try to correct it later during a spe cific pronunciation class, the student tends to forget and deny the mistake he committed. Thus, pronunciation should not be treated separately but should be taught all throughout. For beginners, I teach pronunciation separately during the first lessons. Of course it would be irrational to be teaching beginners many words that would just confuse them and worst, might scare them. Thus, I introduce my lessons systematically starting with the basic and common sounds and even giving examples to magnify

Western Blot and its Applications Essay Example | Topics and Well Written Essays - 1000 words

Western Blot and its Applications - Essay Example Western blot analysis can detect your protein of interest from a mixture of a great number of proteins. It can give you information about the size of your protein (with comparison to a size marker or ladder in kDa), and also give you information on protein expression (with comparison to a control such as untreated sample or another cell type or tissue). It can analyze any protein sample whether from cells or tissues, but also can analyze recombinant proteins synthesized in vitro.Western blot is dependent on the quality of antibody you use to probe for your protein of interest, and how specific it is for this protein. In western blotting, proteins are electrophoresed in polyacrylamide gel, transferred onto a nitrocellulose or nylon membrane, and the protein bands are detected by their specific interaction with antibodies, lectins or some other compounds. First step involves the separation of tissue. Then proteins are separated by gel electrophoresis from sample. If you are going to western blot for protein mass you can lyse in larger volumes If you are going to western blot a phospho-protein use phosphatase inhibitors. It will remove the phosphates from your proteins. If you are looking at protein-protein interactions use a less-stringent detergent such RIPA. By far the most common type of gel electrophoresis employs polyacrylamide gels and buffers loaded with Sodium Dodecyl Sulphate (SDS). SDS PAGE allows separation of proteins by their molecular weight. Steps involved in obtaining protein for western blot. The protein bands are transferred onto a nitrocellulose or nylon membrane; initially this was achieved by a capillary movement of buffer but now a days it is usually done by electrophoresis (electrophoretic blotting). The electrophoretic blotting, both of proteins and nucleic acids are much faster and more efficient than capillary blotting. One thing to be aware of is that proteins bind better to nitrocellulose at a low pH. You may need to go through some trial-and-error to find the optimal pH. You also need to be sure there are no air bubbles between the nitrocellulose and the gel or your proteins will not transfer. The specific protein bands are identified in a variety of ways. 1. Antibodies are the most commonly used probes for detecting specific antigens. 2. Lectins are used as probes for the identification of glycoproteins. These probes may themselves be radioactive or a radioactive molecule may be tagged to them. Often the identification process is based on a 'Sandwich' reaction. In such an approach, a species specific second antibody or protein A of Staphylococcus aureus or streptavidin is used to bind to the antibodies bound to the protein bands. These second molecules may be labelled with radioactive, enzyme or fluorescent tags, Steps in conducting a western blot. a single preparation of these labelled molecules can be employed as a general detector for various probes. Applications in different fields The conformity HIV test uses a HIV western blot to detect anti- HIV. The HIV Western blot consists of a thin nitrocellulose strip in which are embedded proteins claimed to be unique to HIV. Each protein is labelled with a 'p' followed by its molecular weight in thousands. Serum is added to the strip and if there are antibodies to a particular protein this band will 'light up'. The HIV Western blot is not standardised. Lyme disease testing also uses Western blotting. The IgG Western Blot is a sandwich-type immunoassay performed in a manner that allows visualization of the patient's antibodies. It is a qualitative test and is generally more sensitive and specific than the ELISA. A positive IgG result with clinical history may be indicative of

Coalition for Environmentally Responsible Economies (CERES)

Coalition for Environmentally Responsible Economies (CERES) Coalition for Environmentally Responsible Economies (CERES), a non-profit organization was founded in 1989. Its objectives include encouraging ethical and sustainable business practices and promoting investment policies which are environmentally, socially and financially sound. It has created a group of worlds largest companies and investors who are responsible to take care of the environmental issues. 100-plus companies and many Fortune 500 businesses like- General Motors, Polaroid, Body Shop International, Bank of America, are engaged with Ceres for supporting clean energy policies and maintaining a strong climate. Financial organizations and Environmental groups like Sierra Club are members with Ceres (Coalition for Environmentally Responsible Economies (CERES), 2016).   Ceres aims at making companies understand the concerns of the planets future. Meeting the sustainable challenges of the 21st century is important along with running business. Ceres works on making the environment more sustainable and therefore it follows all the laws that comes under the Environmental Laws and Regulations of the U.S. government. Ceres rights: On the account of the Exxon Valdez Oil Spill that happened in the Gulf of Alaska in March 1989, Ceres came up with its core Ten Principles. These principles aim to protect the earth, its energy and resources wisely; reduce waste and doing business in fair manner. The principles and its aspects are as follows: (The Ceres Principles , n.d.) Laws supported by Ceres- There are several issues addressed by U.S. Environmental Protection Agency(EPA). Some of them include- limitation on air pollutants, enforcement of clean water and laws on safe drinking. EPA works for implementation of these federal laws so that the environment does not get affected by business practices. Ceres works along with EPA in implementation of the laws. The INCR Policy Working Group an initiative by Ceres focuses on five different areas of policy engagement: Its members collaborate with other investor groups, including the European IIGCC, the Australian/New Zealand IGCC, the Asian AIGCC, UN PRI and UNEP-FI, to support the Strong Climate and Clean Energy Policy among the investors worldwide. Its members also support the policies which promote clean and low carbon fuel sources, improving Corporate Average Fuel Economy (CAFE) standards, and reduction of greenhouse gas emissions which happen by transportation activities. INCR members also support research reports which show the investment case for such policies. Under the Clean Air Act, the EPA has proposed an arrangement of noteworthy controls that will lessen air emissions, help the economy and make occupations. Financial specialists have signed letters and met with Congressional staff to guarantee implementation of the Utility Mercury and Air Toxics Standard, the Cross State Air Pollution Rule and the Carbon Pollution Standard for Future Power Plants. The Ceres Roadmap to Sustainability, organizations are relied upon to adjust publicly disclosed arrangement positions on atmosphere and energy issues with the approaches they advocate through their lobbying and political expenditures. Its members bolster a scope of approaches at the state and government level, for example, the Production Tax Credit for renewable vitality, the California AB32 climate and Clean Energy Program and the Regional Greenhouse Gas Initiative (RGGI). Ceres makes sure companies and investors follow the strong climate and Clean energy policies in the US and abroad. One of the CEOs of Ceres- Mindy S. Lubber joined U.S. Environmental Protection Agency (EPA) in 1995. She joined it as a senior policy advisor and has achieved recognition as a regional administration under President Bill Clinton in 2000. She has also been a president at former National Environmental Law Center. Her efforts include- Organizing waste removal at hazardous waste sites Redevelopment, new jobs and urban revitalization Protection of drinking water supplies (2016 William K. Reilly Award Winners Announced, n.d.). Ceres along with WWF assembled a group of 100 large companies which supported the Paris Climate Agreement. It also supports the Clean Power Plant which was an initiative by Obama administration. Ceres strongly supports Clean Air Act(Business Is Taking Action on LGBT Rights. Will Climate Change Be Next?, n.d.). In the Six Proactive steps that Ceres has made for insurance companies, for addressing the climate change, it mentions about the Greenhouse gas emission, in favor of Federal and International Policies which limit its emissions. It addresses the threat of change in the climate because of carbon pollution (Insurance, n.d.). Laws opposed by Ceres- Ceres mostly works for sustaining the environment and limiting business practices for better future. It supports laws that relate to maintaining environment standards. Therefore, there arent many laws that are being opposed by Ceres. Rather there are a couple of them which are addressed as below. It opposed the Presidents and Congressional Leaders abandoning of Methane rule and the Clean Power Plant. Ceres believes that these laws were made to support the environment. There are many companies that has made an agreement in support of the law. And so, Ceres does not want such laws to get abandoned. It also shows its disagreement with the Presidents two-for-one executive order which requires deletion of two federal regulations against any one issued. Ceres impact on business- Ceres major goal lies in influencing business and its practices which affect environment. It has made many huge contributions which has helped business to follow the standards and become effective in their practices. The contributions by Ceres which impacted companies include- Investor Network on Climate Risk (INCR), 2003- works on leadership and accomplishments on climate and sustainability of financial firms. Launched the Global Reporting Initiative (GRI) for corporate reporting on environment, social and economic performances Launched the Climate Declaration Launched Business for Innovative Climate and Energy Policy (BICEP) Ceres made an evaluation of its member companies and found that most of them have adopted policies based on International norms and fewer companies have adopted sector-specific policies. In the evaluation, most frequent instituted policies include those related to bribery and corruption and elimination of discrimination along with overall sustainability of environment. Companies which adopted the international standards like International Labor Organization(ILO) and United Nations Global Compact (UNGC), show understanding of sustainability issues and are committed in implementing changes in their business (Corporate Policies and Management Systems, n.d.). Ceres influence on legislative process- Ceres resolved an issue on United Parcel Service (UPS)s Lobbying Disclosure. It laid down points which the BOD of UPS requires to disclose in their report. Indirect lobbying and Grassroots lobbying communication are the points covered under the solution. These points include efforts at the local, state and federal levels. Along with this, we already saw that Ceres has laid down its core 10 principles which are in favor of environmental protection. Business and stakeholders adhere to these aspects and make sure that they are abiding by the rules related to these policies. It tracks shareholders resolutions and considers the following issues: Climate change Carbon asset risk Greenhouse gas emission Water pollution and scarcity Public policy Air pollution methane emission Proxy voting policies Human rights The shareholders make such resolutions. This ensures that they make proper implementation of the laws. On studying the resolutions, the legislatures can make laws that depend upon the environmental standards. Ceres 3-year plan- Ceres has presented a Road map on Sustainability which covers Governance for sustainability, Stakeholder Engagement, Disclosure, Performance: Operation, Supply Chain, Transportation and Logistics, Product and Services, Employees. The Roadmap expectation on corporate policies and management systems measures the extent to which companies embed sustainability into decision-making. Encourage organizations to receive a more expansive way to deal with recognizing and speaking with stakeholders, including engagement on a more extensive topic. The disclosure segment layout the attributes of a way to deal with revelation that meets the new and rising difficulties on corporate sustainability. The operation segment enhances sustainability across their operations, including building and facilities management, water management, the elimination of waste and respect for human rights. Sustainable supply chain performance expects establishing supplier policies and endorsing industry codes or practices containing explicit references to social and environmental standards. Transportation and logistics include reducing transportation impacts which are focused on the architecture of the transportation network, including distances traveled; and specific transportation modes, including the sustainability credentials of the energy sources used. This section also looks at business travel and commuting practices and the role that companies can take in shaping employee behavior. Sustainable solutions for products and services should be that they are marketed and delivered in a sustainable way. Proper communication of the usage and consumption pattern. Improved recruitment and retention rates, employee morale and productivity, and lower healthcare costs should be crucial for companys employees in order for sustainable performance (The Ceres Roadmap for Sustainability Ceres, n.d.). Ceres aims at transferring the way corporations integrate environmental and social risks into their decision-making. By 2020 Ceres wants major companies which re its members to attain these standards. The areas in which more companies should come forward include Product and Services and Supply Chain. Ceres looks forward in making greater attempts at helping companies in achieving the goals of sustainable environmental business practices. Mind-Map: Ceres an organization works majorly in line with the International Laws on Environmental protection. It supports U.S. laws as well about certain policies. Ceres helps implementation of the following laws of Unites States Federal Environmental Statutes- Clean air act Clean water act Emergency planning and community right to know act Energy policy act National environmental policy act Resources conservation and recovery act References (n.d.). Retrieved from 2016 William K. Reilly Award Winners Announced: http://www.american.edu/spa/cep/news/2016_Reilly_Award_Winners.cfm Business Is Taking Action on LGBT Rights. Will Climate Change Be Next? (n.d.). Retrieved from Business Is Taking Action on LGBT Rights. Will Climate Change Be Next? Ceres: https://www.ceres.org/press/press-clips/business-is-taking-action-on-lgbt-rights.-will-climate-change-be-next Coalition for Environmentally Responsible Economies (CERES). (2016, november 24). Retrieved from Encyclopà ¦dia Britannica: https://www.britannica.com/topic/Coalition-for-Environmentally-Responsible-Economies Corporate Policies and Management Systems. (n.d.). Retrieved from Corporate Policies and Management Systems Ceres: https://www.ceres.org/roadmap-assessment/about/roadmap-expectations/governance-for-sustainability/policies-and-management-systems Insurance. (n.d.). Retrieved from Insurance-Ceres: https://www.ceres.org/industry-initiatives/insurance The Ceres Principles . (n.d.). Retrieved from The Ceres Principles- Ceres: https://www.ceres.org/about-us/our-history/ceres-principles The Ceres Roadmap for Sustainability Ceres. (n.d.). Retrieved from The Ceres Roadmap for Sustainability Ceres: https://www.ceres.org/roadmap-assessment

First Impressions in Bernard Shaw’s play The Devil’s Disciple Essay

Bernard Shaw’s play â€Å"The Devil’s Disciple† is set in 1777 when the American War of British Independence was at its height. Richard Dudgeon is the main character in this production and he begins as a ‘black sheep’ in a family of self-righteous puritans. Richard has entitled himself â€Å"The Devil’s Disciple†. In the first Act of the play, Richard’s father has died and this starts the reading of his will. During this scene we get a lot of the other characters views on Richard before we meet him. It seems that almost everyone apart from Essie (Richard’s cousin). Everyone finds it hard to talk about him as if it is against the rules, e.g. Judith Anderson says to Essie, â€Å"Dick Dudgeon! Essie: do you wish to be a really respectable and grateful girl, and to make a place for yourself here by steady and good conduct?† †Then you must never mention the name of Richard Dudgeon – never think even about him. He is a bad man†. This suggests that Judith believes even thinking about Richard could mean you were becoming a bad person yourself. However, we find out later that she has never actually met Richard Dudgeon, so is only saying this from what she has heard. Whilst Mrs Dudgeon and Mr Anderson are talking at the very beginning of the scene, Mrs Dudgeon also expresses her view of Richard. She says, â€Å"Let it be a warning to him. He may end that way himself, the wicked, dissolute, godless—â€Å" This shows us that he is even hated by his own mother. From all of the views shown in the early stages of the first act about Richard, we get the impression that he is a terrible person, with no respect or cares for anyone else. Even when we meet Richard, our opinions as an audience don’t change greatly, he shows himself as quite arrogant and sarcastic. How... ... a fool. Judith: Like a hero. I think that Shaw made us change our opinion of Richard in the context of the story that he is telling because it shows us how even the ‘worst’ people among us can change, or that these people are not so bad after all, just covering up their true selves until the time is right. Anderson (whilst talking to the British soldiers) says, â€Å"Sir: it is in the hour of trial that a man finds his true profession. This foolish young man [placing his hand on Richard's shoulder] boasted himself the Devil's Disciple, but when the hour of trial came to him, he found that it was his destiny to suffer and be faithful to the death. I thought myself a decent minister of the gospel of peace, but when the hour of trial came to me, I found that it was my destiny to be a man of action and that my place was amid the thunder of the captains and the shouting.†